修饰抗体 Products
组蛋白H2A抗体
杭州景杰生物科技有限公司
Histone H2A.x mouse mAb

Cat#:

PTM-1005

Ab Type:

M

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description

This product is produced by immunizing mice with a synthetic peptide corresponding to C terminus of human histone H2A.x. Antibodies are purified by peptide affinity chromatography.


Source/Purification

This product is produced by immunizing mice with a synthetic peptide corresponding to C terminus of human histone H2A.x. Antibodies are purified by peptide affinity chromatography.


Specificity

H2A.x

Recommended Applications

ELISA,WB; Not tested in other applications

*WB=Western blotting;


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Histone H2A.x mouse mAb

A. Western blotting analysis on 30 μg of crude proteins from human MCF-7, HeLa, H1299, JK whole cell lysates using histone H2A.x mouse mAb (1:2000).


B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of nocodazole (100 ng/ml, 18hr) and calyculin A (100 nM, 1hr) using histone H2A.x mouse mAb (1:2000).The membrane was re-probed by anti-phospho-histone H2A.x (Ser139) mouse mAb.


Scientific Description

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H4 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.


Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**This product is intended for research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.


ELISA,WB; Not tested in other applications

*WB=Western blotting;



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