修饰抗体 Products
组蛋白赖氨酸丙二酰化(malonylation)修饰抗体
杭州景杰生物科技有限公司
Anti-malonyl-Histone H3 (Lys122) rabbit pAb

Cat#:PTM-910Ab Type:P

Ab Size:100 μlApplications:WB

Species:H M RPrice:¥ 3780.00


Product Description
The rabbit-derived antibody is purified with protein A-conjugated agarose followed by malonylated histone H3 (Lys122) peptide affinity chromatography. It specifically recognizes histone H3 with malonylation at Lys122.  
Source/Purification
This product is produced by immunizing rabbits with a synthetic malonyl peptide corresponding to residues surrounding Lys122 of human histone H3. Antibodies are purified by protein A-conjugated agarose followed by malonylation histone H3 (Lys122) peptide affinity chromatography.   
Specificity

H3

Recommended Applications
ELISA, WB; Not tested in other applications  
*WB=Western blotting;  
Recommended antibody dilution

WB: 1:2000

Important

  Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.  

 

Anti-malonyl-Histone H3 (Lys122) rabbit pAb

ADot blotting analysis on indicated amount of malonylated H3 peptide at Lys122 (lane 1), acetylated H3 peptide at Lys122 (lane 2), butyrylated H3 peptide at Lys122 (lane 3), crotonylated H3 peptide at Lys122 (lane 4), succinylated H3 peptide at Lys122 (lane 5), 2ohibutyrylated H3 peptide at Lys122 (lane 6), and unmodified H3 peptide at Lys122 (lane 7) using malonyl-histone H3 (Lys122) rabbit pAb.   

 

B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium malonate (50 mM, 72 hours) using malonyl-histone H3 (Lys122) rabbit pAb (1:2000).  

 

Scientific Description
Malonylation of lysine, a newly identified reversible modification,controls protein activity. Sirt5, a regulatory enzyme for lysine malonylation, catalyzes lysine de-malonylation reactions in vivo and in vitro. Integrated proteomic  and biochemistry analyses demonstrated thatlysine malonylation presents in both prokaryotes and eukaryotes in wide ranges of proteins including histones and non-histone substrates. Lysine malonylation may provide a new paradigm in the cellular regulation, such as energy metabolism in diverse organisms.  
Storage & Stability

The antibody is delivered in powder form. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 


**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


ELISA, WB; Not tested in other applications  
*WB=Western blotting;  


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