修饰抗体 Products
组蛋白精氨酸胍基化(citrullinate)修饰抗体
杭州景杰生物科技有限公司
Anti-cit-histone H2A (Arg3) rabbit pAb

Cat#:

PTM-1305

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

2500

Price: (20 μl)

750.00


Product Description
Source/Purification

This product is produced by immunizing rabbits with a synthetic citrullinated peptide corresponding to residues surrounding Arg3 of human Histone H2A. Antibodies are purified by protein A-conjugated agarose followed by citrullinated Histone H2A peptide affinity chromatography


Specificity

H2A

Recommended Applications

For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications 

Recommended antibody dilution

/

Important

 For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

 Use at an assay dependent concentration.  Optimal dilutions/concentrations should be determined by the end user.  

捕获6.JPG

捕获7.JPG 

 A.Dot blotting analysis on indicated amount of peptides using histone H2AR3cit rabbit pAb. The list of peptides is included in the table on the right.

 

 B.Western blotting analysis on 30 μg of crude proteins from HL-60 whole cell lysates with (right) or without (left) treatment of calcium Ionophore (4 μM, 15 min) using citrul-Histone H2A (Arg 3) rabbit pAb (1:2000).

Scientific Description

Protein citrullination is an emerging PTM that results from the conversion of peptidyl arginine to peptidyl citrulline. Due to the exchange of an imine for a carbonyl group, this reaction is referred to as deimination. This PTM is catalyzed by the calcium-regulated PAD family of enzymes. Dysregulated PAD expression and aberrant protein citrullination have been implicated in numerous human diseases such as osteosarcoma, colorectal cancer, esophagus cancer and breast cancer. 


Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.


For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications 

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