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赖氨酸琥珀酰化 (succinylation) 抗体
杭州景杰生物科技有限公司
Anti-succinyllysine rabbit pAb

Cat#:PTM-401

Ab Size:100 μl   

Species:All

Ab Type:P

Applications:WB IP

Price:¥ 3680.00


Product Description

The rabbit-derived pan lysine succinylated antibody is purified by succinyl-lysine agarose affinity chromatography. It specifically recognizes proteins with lysine succinylated residues but not the acetyllysine or non-modified lysine residues. Besides common applications in Western blotting, immunoprecipitation and immunofluorescence, the antibody has been well utilized for proteomic screening to lysine succinylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


Source/Purification

This product is produced by immunizing rabbits with succinylated BSA. Antibodies are purified by succinylated-lysine agarose affinity chromatography.  


Specificity

All

Recommended Applications

ELISA, WB, IP   

*WB=Western blotting; IP=Immunopreciptation 


Recommended antibody dilution

WB: 1:1000; IP: 1: 25-1: 50

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user. 

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Scientific Description

Lysine succinylation is a recently identified novel Protein Post-translational Modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both eukaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function.


Storage & Stability

Store product at -20℃. Avoid repeated freeze / thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.


**This product is intended for research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



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ELISA, WB, IP   

*WB=Western blotting; IP=Immunopreciptation 


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