修饰抗体 Products
组蛋白赖氨酸乙酰化 (acetylation) 修饰抗体
杭州景杰生物科技有限公司
Acetyl-p53 (Lys139) mouse mAb

Cat#:

PTM-185

Ab Type:

M

Application:

WB, Dot, ELISA

Species:

Human, Mouse, Rat, 

Price: (100ul)

3060

Price: (20ul)

700

Source/Purification:  

This product is produced by immunizing mice with a synthetic acetyl peptide corresponding to residues surrounding Lys139 of human p53. Antibodies are purified by protein G-conjugated agarose followed by

acetylated p53 (Lys139) peptide affinity chromatography.


Recommended Applications: 

ELISA, Dot blot, Western blot.  Recommended antibody dilution: WB: 1:2000

NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications


                           

Scientific Description:

 

Acetyl-p53 (Lys139) mouse mAb

Figure A: Dot blotting analysis on indicated amount of peptides using anti-p53K139ac mouse mAb. The list of peptides is included in the table on the right.

Acetyl-p53 (Lys139) mouse mAb

Figure B: Western blotting analysis on 30 μg of crude proteins from human MCF-7 whole cell lysates with (right) or without (left) treatment of etoposide (15ug/ml, 24 hours) and (500ng/ml, 24 hours)Trichostatin A using acetyl-p53 (Lys139) mouse mAb (1: 2000).

Acetyl-p53 (Lys139) mouse mAb

Figure C: Western blotting analysis on 30 μg of crude proteins from mouse brain and mouse liver whole tissue lysates using acetyl-p53 (Lys139) mouse mAb (1: 2000).


Storage & Stability:

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.

ELISA, Dot blot, Western blot. 

Recommended antibody dilution: WB: 1:2000



推荐产品 PRODUCTS
Copyright ©2018-2020 杭州景杰生物科技有限公司
犀牛云提供云计算服务
X
合作
交流
1

QQ设置

3

SKYPE 设置

4

阿里旺旺设置

等待加载动态数据...

等待加载动态数据...

5

电话号码管理

  • 400-100-1145
6

二维码管理

等待加载动态数据...

等待加载动态数据...

展开
合作交流
填写信息,我们的专属学术顾问将为您解答疑问!