修饰抗体 Products
组蛋白精氨酸甲基化 (arg-methylation) 修饰抗体
杭州景杰生物科技有限公司
Anti-di-methyl(sy)-Histone H4 (Arg3) rabbit pAb

Cat#:

PTM-639

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

3060.00

Price: (20 μl)

700.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by symmetric di-methylated histone H4 (Arg3) peptide affinity chromatography. It specifically recognizes histone H4 with symmetric di-methylation at Arg3.

Source/Purification

This product is produced by immunizing rabbits with a synthetic symmetric di-methyl peptide corresponding to residues surrounding Arg3 of human histone H4. Antibodies are purified by protein A-conjugated agarose followed by symmetric di-methylated histone H4 (Arg3) peptide affinity chromatography. 

Specificity

H3

Recommended Applications

ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting; IP= Immunoprecipation


Recommended antibody dilution

WB: 1:2000 IP: 1:50

Important

For Western blotting,  incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1%  Tween-20 for two hours at room temperature with gentle shaking.  

Use at an assay dependent  concentration. Optimal dilutions/concentrations should be determined by the end  user.   

Anti-di-methyl(sy)-Histone H4 (Arg3) rabbit pAb

A. Dot  blotting analysis on indicated amount of symmetric di-methylated H4 peptide at Arg3  (lane 1), asymmetric di-methylated H4 peptide at Arg3 (lane2), acetylated H4  peptide at Lys5 (lane3) and non-methylated H4 peptide at Arg3 (lane 4) using symmetric  di-methyl-histone H4 (Arg3) rabbit pAb.

 

B. Western blotting analysis on 30 μg of crude proteins  from HeLa, A431, C2C12, AML-12 and COS whole cell lysates using symmetric di-methyl-histone  H4 (Arg3) rabbit pAb (1:2000).  



Scientific Description

Proteins post-translational Modification (PTM) is an important reversible event controlling protein activity. The amino-terminal tails of core histones undergo multiple modifications in multiple sites, termed as “histone code” or “epigenetic code”. Lysine methylation or arginine methylation in core histones is a major determinant for the formation of active and inactive regions of the genome and therefore plays vital roles in multiple cellular events. In most species, arginine methylation occurs primarily on histones H3 (Arg2, 8, 17, 26) and H4 (Arg3) and has been implicated in both transcriptional activation and silencing. Arginine methylation in histones is carried out by the arginine N-methyltransferase (PRMT), which catalyzes the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine. Arginine methylation in histones is impaired in the pathologies of cancer and other diseases and therefore, enzymes regulating histone arginine methylation have become promising targets for anti-cancer drugs.  

Storage & Stability

Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


**Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



  1. Mavrakis, K.J. et al, Science. 2016 Mar 11; 351(6278):1208-13.  

ELISA, WB, IP; Not tested in other applications  

*WB=Western blotting; IP= Immunoprecipation


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