修饰抗体 Products
组蛋白H2A抗体
杭州景杰生物科技有限公司
Anti-histone H2A rabbit pAb

Cat#:

PTM-1008

Ab Type:

P

Applications:

WB, Dot, ELISA

Species:

Human, Mouse, Rat

Price: (100 μl)

1380.00

Price: (20 μl)

414.00


Product Description

The rabbit-derived antibody is purified with protein A-conjugated agarose followed by affinity chromatography on immunogen peptide. It specifically recognizes endogenous histone H2A protein and doesnot cross-react with other histones.   


Source/Purification

This product is produced by immunizing rabbits with a synthetic peptide corresponding to C terminus of human histone H2A. Antibodies are purified by peptide affinity chromatography.  


Specificity

H2A

Recommended Applications

ELISA, WB; Not tested in other applications  

*WB=Western blotting;  


Recommended antibody dilution

WB: 1:2000

Important

For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user.  

Anti-histone H2A rabbit pAb

A. Western blotting analysis on 30 μg of crude proteins from HeLa, COS-7, BRL whole cell lysates, and Liver, Kidney tissue lysate using histone H2A rabbit pAb (1:2000).   


B. Western blotting analysis on 30 μg of crude proteins from HeLa whole cell lysates with (right) or without (left) treatment of sodium butyrate (30 mM, 4hr) using histone H2A rabbit pAb (1:2000).The membrane was re-probed by anti-acetyl-histone H2A (Lys5) rabbit pAb (1:2000).  


Scientific Description

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries. Histone H2A helps regulate chromatin structure and function through post-translational modifications and specialized histone variants. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.  


Storage & Stability

The antibody is delivered in powder form. After dissolved with the antibody reconstitution buffer, please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. 

 

**For research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


ELISA, WB; Not tested in other applications  

*WB=Western blotting;  


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