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Anti-diglycyllysine rabbit pAb

  • 服务简介

    Product Description

    The anti-diglycine lysine antibody (anti-K-ɛ-G-G antibody) is produced by immunizing rabbits with conjugates of K-ɛ-GG-KLH. Antibodies are purified by diglycine lysine agarose affinity chromatography. The product specifically recognizes proteins and peptides with Gly-Gly sequences that are present as an adduct on the ?-amine of lysine. This antibody has been well utilized as affinity reagents for proteomic screening of lysine ubiquitinated peptides from trypsin-digested proteins both in eukaryotic and prokaryotic cells.


    Source/Purification

    The anti-diglycine lysine antibody (anti-K-ɛ-G-G antibody) is produced by immunizing rabbits with conjugates of K-ɛ-GG-KLH. Antibodies are purified by diglycine lysine agarose affinity chromatography.


    Specificity

    All

    Recommended Applications

    IAC  IAC=Immunoaffinity Immunoprecipitation

     

    Recommended antibody dilution

    /

    Important

    Only for IP-MS,not for WB or other application.

         

    Anti-diglycyllysine rabbit pAb

    A. Dot blotting analysis on indicated amount of K-ɛ-GG-KLH (lane 1), K-ɛ-GG peptide library (lane 2),  synthetic unmodified human histone H3 peptide STGGKAPR (aa 10-17) (lane 3),  KLH (lane 4) and control peptide of K-ɛGG peptide library (lane 5, unmodified lysine peptide  library) using anti-diglycine lysine rabbit pAb (1:2000).

     

    Anti-diglycyllysine rabbit pAb

    Anti-diglycyllysine rabbit pAb

    B. Mass spectrometer analysis: Representative annotated MS/MS spectra of an  ubiquitin remnant-containing peptide.  Crude proteins from  HeLa cell lysate were digested  by trypsin. The ubiquitin remnant-containing peptides were affinity  enriched by the anti-diglycine lysine rabbit pAb followed by LC-ESI-MS/MS  in a Q-Exactive mass spectrometry. The sequence of the representative ubiquitinated  peptide is indicated and the fragment ions are labeled in the MS/MS  spectrum.  The diglycine-modified lysine and its position in the peptide  sequence are indicated with “gl” above 'K'.


    Scientific Description

    Ubiquitin(Ub) is a highly conserved protein of 8 kDa that covalently attaches to lysine residues of target proteins. This occurs through a three-step process involving Ub-activating (E1), Ub-conjugating (E2) and Ub-ligating (E3) enzymes. Ubiquitin-dependent processes are commonly modulated via formation of polyubiquitin chains, whereby the C terminus of a chain extending ubiquitin becomes linked to the N terminus or one of seven lysine residues (Lys-6,Lys-11, Lys-27, Lys-29, Lys-33, Lys-48, and Lys-63) within a substrate-bound ubiquitin molecule. The modification of lysine ubiquitin (ubiquitination) plays a central role in the regulation of multiple cellular processes. In addition to targeting proteins for proteasomal degradation, ubiquitination modulates membrane protein trafficking, alters protein-protein interactions, and controls the activity of many signal transduction pathways. A large family of enzymes involved in ubiquitin conjugation controls these diverse functions.


    Storage & Stability

    Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


    **Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



  • 技术原理

    Cat#:

    PTM-1101

    Ab Type:

    P

    Applications:

    IAC

    Species:

    All

    Price: (100 μl)

    3360.00

    Price: (20 μl)

    1008.00


  • 仪器设备

  • 技术优势

    1. Guoqiang, X., et al. (2010). Nat Biotech. 28:868-873;

    2. Daisy, B., et al. (2012). Mol.Cell.Pro. 2012, 11: 1529-1540.


  • 应用领域

    IAC  IAC=Immunoaffinity Immunoprecipitation

  • 参考文献

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