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Anti-succinyllysine rabbit pAb

  • 服务简介

    Product Description

    The rabbit-derived pan lysine succinylated antibody is purified by succinyl-lysine agarose affinity chromatography. It specifically recognizes proteins with lysine succinylated residues but not the acetyllysine or non-modified lysine residues. Besides common applications in Western blotting, immunoprecipitation and immunofluorescence, the antibody has been well utilized for proteomic screening to lysine succinylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


    Source/Purification

    This product is produced by immunizing rabbits with succinylated BSA. Antibodies are purified by succinylated-lysine agarose affinity chromatography.  


    Specificity

    All

    Recommended Applications

    ELISA, WB, IP   

    *WB=Western blotting; IP=Immunopreciptation 


    Recommended antibody dilution

    WB: 1:1000; IP: 1: 25-1: 50

    Important

    For Western blotting, incubate  membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for  two hours at room temperature with gentle shaking.  

    Use at an assay dependent concentration. Optimal  dilutions/concentrations should be determined by the end user. 

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    Scientific Description

    Lysine succinylation is a recently identified novel Protein Post-translational Modification (PTM). Lysine succinylation is evolutionarily conserved and abundantly present in proteins in both eukaryotic and eukaryotic cells, suggesting its roles in regulating cellular physiology. This antibody will facilitate studies of lysine succinylation biology by identifying its substrates and studying its function.


    Storage & Stability

    Store product at -20℃. Avoid repeated freeze / thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide. Stable for 12 months from date of receipt.


    **This product is intended for research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



  • 技术原理

    Cat#:

    PTM-401

    Ab Type:

    P

    Applications:

    WB

    Species:

    All

    Price: (100 μl)

    3680.00

    Price: (20 μl)

    1104.00


  • 仪器设备

  • 技术优势

    1. Zhang Z, Tan M, Xie Z, et al. (2011) Nat Chem Biol. 201, 7(1):58-63.  

    2. Peng C, Liu Z, Xie Z, et al. (2011) Molecular and Celluar Proteomics (Epub ahead of print)  

    3. Michael H. Schwartz, Jacob R, et al. (2016) Nucleic Acids Research. 2016 1 doi: 10.1093/nar/gkw856.  

    4. Zhou L,S, Wang F, Sun R,Q, et al. Scientific Reports. 2016, 17(6):811-22.  

    5. Colak G,et al.Identification of lysine succinylation substrates and the succinylation regulatory enzyme CobB in Escherichia coli.Mol Cell Proteomics. 2013 Dec;12(12):3509-20. doi: 10.1074/mcp.M113.031567. Epub 2013 Oct 31.

    6. Park J,et al.SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.Mol Cell. 2013 Jun 27;50(6):919-30. doi: 10.1016/j.molcel.2013.06.001.

    7.  Weinert BT,et al.Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation.Cell Rep. 2013 Aug 29;4(4):842-51. doi: 10.1016/j.celrep.2013.07.024. Epub 2013 Aug 15.

    8.  Zhang Z,et al.Identification of lysine succinylation as a new post-translational modification.Nat Chem Biol. 2011 Jan;7(1):58-63. doi: 10.1038/nchembio.495. Epub 2010 Dec 12.

    9. Xie Z,et al. Lysine succinylation and lysine malonylation in histones.Mol Cell Proteomics. 2012 May;11(5):100-7. doi: 10.1074/mcp.M111.015875. Epub 2012 Mar 4.

    10. Tannahill GM,et al.Succinate is an inflammatory signal that induces IL-1β through HIF-1α.Nature. 2013 Apr 11;496(7444):238-42. doi: 10.1038/nature11986. Epub 2013 Mar 24.

    11. Yu J,et al. Metabolic characterization of a Sirt5 deficient mouse model.Sci Rep. 2013 Sep 30;3:2806. doi: 10.1038/srep02806.

    12.Fritz KS,et al.Ethanol metabolism modifies hepatic protein acylation in mice.PLoS One. 2013 Sep 20;8(9):e75868. doi: 10.1371/journal.pone.0075868. eCollection 2013.

    13. Wagner GR,et al.Widespread and enzyme-independent Nε-acetylation and Nε-succinylation of proteins in the chemical conditions of the mitochondrial matrix.J Biol Chem. 2013 Oct 4;288(40):29036-45. doi: 10.1074/jbc.M113.486753. Epub 2013 Aug 13.

    14. Lin ZF,et al.SIRT5 desuccinylates and activates SOD1 to eliminate ROS.Biochem Biophys Res Commun. 2013 Nov 8;441(1):191-5. doi: 10.1016/j.bbrc.2013.10.033. Epub 2013 Oct 16.

    15. Clinical Lung Cancer Genome Project (CLCGP); Network Genomic Medicine (NGM).A genomics-based classification of human lung tumors.Sci Transl Med. 2013 Oct 30;5(209):209ra153. doi: 10.1126/scitranslmed.3006802.

    16. Manuel AM,et al.Adipocyte protein modification by Krebs cycle intermediates and fumarate ester-derived succination.Amino Acids. 2013 Nov;45(5):1243-7. doi: 10.1007/s00726-013-1568-z. Epub 2013 Jul 28.

    17. Nardelli SC,et al.The histone code of Toxoplasma gondii comprises conserved and unique posttranslational modifications.MBio. 2013 Dec 10;4(6):e00922-13. doi: 10.1128/mBio.00922-13.

    18. Buler M,et al.SIRT5 is under the control of PGC-1α and AMPK and is involved in regulation of mitochondrial energy metabolism.FASEB J. 2014 Jul;28(7):3225-37. doi: 10.1096/fj.13-245241. Epub 2014 Mar 31.

    19. Yang M,et al.Succinylome analysis reveals the involvement of lysine succinylation in metabolism in pathogenic Mycobacterium tuberculosis.Mol Cell Proteomics. 2015 Apr;14(4):796-811. doi: 10.1074/mcp.M114.045922. Epub 2015 Jan 20.

    20. Du Y,et al.Lysine malonylation is elevated in type 2 diabetic mouse models and enriched in metabolic associated proteins.Mol Cell Proteomics. 2015 Jan;14(1):227-36. doi: 10.1074/mcp.M114.041947. Epub 2014 Nov 23.

    21. Polletta L,et al.SIRT5 regulation of ammonia-induced autophagy and mitophagy.Autophagy. 2015;11(2):253-70. doi: 10.1080/15548627.2015.1009778.

    22.Madsen CT,et al.Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p.Nat Commun. 2015 Jul 9;6:7726. doi: 10.1038/ncomms8726.

    23.Singhal A,et al.Systematic Analysis of Mycobacterial Acylation Reveals First Example of Acylation-mediated Regulation of Enzyme Activity of a Bacterial Phosphatase.J Biol Chem. 2015 Oct 23;290(43):26218-34. doi: 10.1074/jbc.M115.687269. Epub 2015 Sep 8.

    24.Boylston JA,et al.Characterization of the cardiac succinylome and its role in ischemia-reperfusion injury.J Mol Cell Cardiol. 2015 Nov;88:73-81. doi: 10.1016/j.yjmcc.2015.09.005. Epub 2015 Sep 24.

    25.He D,et al. Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed.J Proteome Res. 2016 Mar 4;15(3):879-90. doi: 10.1021/acs.jproteome.5b00805. Epub 2016 Jan 28.

    26.Sadhukhan S,et al. Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function.Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4320-5. doi: 10.1073/pnas.1519858113. Epub 2016 Apr 5.

    27. Li L,et al.SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability.Nat Commun. 2016 Jul 20;7:12235. doi: 10.1038/ncomms12235.

    28. Fukushima A,et al.Acetylation and succinylation contribute to maturational alterations in energy metabolism in the newborn heart.Am J Physiol Heart Circ Physiol. 2016 Aug 1;311(2):H347-63. doi: 10.1152/ajpheart.00900.2015. Epub 2016 Jun 3.

    29. Lin JB,et al.NAMPT-Mediated NAD(+) Biosynthesis Is Essential for Vision In Mice.Cell Rep. 2016 Sep 27;17(1):69-85. doi: 10.1016/j.celrep.2016.08.073.

    30.Gattu AK,et al.Pigment epithelium-derived factor (PEDF) suppresses IL-1β-mediated c-Jun N-terminal kinase (JNK) activation to improve hepatocyte insulin signaling.Endocrinology. 2014 Apr;155(4):1373-85. doi: 10.1210/en.2013-1785. Epub 2014 Jan 23.


  • 应用领域

    ELISA, WB, IP   

    *WB=Western blotting; IP=Immunopreciptation 


  • 参考文献

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