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Anti-L-lactyllysine rabbit mAb

  • 服务简介

    Product Description

    The pan rabbit monoclonal anti-L-lactyllysine antibody was produced by immunizing rabbits with L-lactylated BSA. Antibodies were purified by protein A. The product specifically recognizes proteins with lysine lactylated residues but not the structurally-similar modified or non-modified lysine residues. The applications of this antibody include Western blotting, immunocytochemistry and immunohistochemistry. In addition, it is well utilized for proteomic screening to lysine lactylated substrates from protease-digested proteins both in prokaryotic and eukaryotic cells.


    Source/purification

    The pan rabbit monoclonal anti-L-lactyllysine antibody was produced by immunizing rabbits with L-lactylated BSA. Antibodies are purified by protein A.



    Specificity

    Anti-L-lactyllysine antibody detects proteins which are post-translationally modified with lactylation at lysine residues, but does not cross-react with the acetylated, propionylated, butyrylated, succinylated, crotonylated and unmodified peptide library.



    Recommended Applications

    ELISA, Dot blot, Western blot, Immunohistochemistry, Immunocytochemistry

    Recommended antibody dilution

    WB: 1:2000, IHC:1:100, ICC/IF: 1:100

    Important NOTES

    For Western blotting, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking.

    Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user.


     

     

     

     

    NO.

    PeptideNO.Peptide

    Anti-L-lactyllysine rabbit mAb

    Lane 1

    Klac library

    Lane 2

    Kac library

    Lane 3

    Kpr library

    Lane 4

    Kbu library

    Lane 5

    Kcr library

    Lane 6

    Kmal library

    Lane 7

    Khib library

    Lane 8

    Ksu library

      Lane 9control library

     

     

     

    Figure A:Dot blotting analysis on indicated amount of peptides using anti-Klac rabbit mAb. The list of peptides is included in the above table.

    Anti-L-lactyllysine rabbit mAb

     

    Figure B: Western blotting analysis on 30 μg   of crude proteins from HeLa whole cell with (+) or without (-) treatment of sodium lactate (100 mM, 24hr) using anti-Kla rabbit mAb (1: 1000).



    Anti-L-lactyllysine rabbit mAb

     

    Anti-L-lactyllysine rabbit mAb

    Figure C: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue, labeling anti-Kla with PTM-1401 RM at 1/100 dilution. 

    Nuclear staining on tumor cells of human colon cancer was observed. Counter stained with hematoxylin.Heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).

     

    Figure D:Immunocytochemical analysis of Raji (Burkitt's lymphoma cell line), labeling anti-Kla with PTM1401RM at 1/100 dilution. Both nuclear and cytoplasmic stainingon Raji cell line. Nuclei were stained with DAPI (blue). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. 


    Scientific Description

    Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation,ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases.Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification,derived from the cellular metabolite lactate.

    Anti-L-lactyllysine rabbit pAb

    Storage & Stability:

    The antibody is delivered in lyophilized powder. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds, and then reconstitute it with the antibody stabilizer provided. The stabilizer contains PBS, 50% glycerol, and 0.01% sodium azide. Please store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Antibody is stable for 12 months from date of reconstitution. Equilibrate the antibody to room temperature for 2 minutes and mix by gently pipetting before use.

    **Research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.



  • 技术原理

    Cat#:

    PTM-1401 RM

    Ab Type:

    rabbit monoclonal

    Applications:

    Price (100 μl):

    WB, IHC, ICC

    ¥4200.00

    Species:


    All species is expected



  • 仪器设备

  • 技术优势

    Zhang D, Tang ZY. et al (2019). Metabolic regulation of gene expression by histone lactylation. Nature, vol. 574, pages 575–580 .

  • 应用领域

    ELISA, WB, IHC, ICC. Not tested in other applications

    *WB=Western blotting; ICC: Immunocytochemistry; IHC=Immunohistochemistry


  • 参考文献

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