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Anti-phosphotyrosine rabbit pAb

  • 服务简介

    Product Description

    Anti-phosphotyrosine rabbit polyclonal antibody was developed by using phosphotyramine-KLH conjugates as immunogen. This antibody can be used in western blotting to examine the modification of tyrosine phosphorylation in immunoprecipitated proteins or the overall profile of tyrosine phosphorylation in cells and tissues.  


    Source/Purification

    The rabbit polyclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides and immunoaffinity purified by protein A followed by phosphotyramine affinity chromatography.  


    Specificity

    All

    Recommended Applications

    ELISA, WB, IAP. Not tested in other applications

    *WB=Western blotting; IAP: Immuno affinity precipitation


    Recommended antibody dilution

    WB: 1:1000; IAP: 1:200

    Important

    For Western blotting, incubate membrane with diluted antibody in 5% BSA, 1 x TBS, 0.1% Tween-20 at 4℃ with gentle shaking, overnight.

    Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications.

    Anti-phosphotyrosine rabbit pAb

    A. Dot blotting analysis on 20 ng of tyrosine phosphorylated peptide library (1A), site specific tyrosine phosphorylated peptides (2A-5A,1B-5B and 1C-4C), threonine phosphorylated peptides (5C and 6A-6C), serine phosphorylated peptides (7A-7C and 8A) and unmodified peptides (8B-8C) using anti-phosphotyrosine rabbit polyclonal antibody . 

    B. Western blotting analysis on 30 µg of crude proteins extracted from HeLa cells with (right) or without (left) sodium vanadate treatment (1 mM for 15 mins) using anti-phosphotyrosine rabbit polyclonal antibody (1:1000).

     

    Scientific Description

    Tyrosine phosphorylation is one of the major means of cell signaling transduction in multiple pathophysiological conditions regulated by tyrosine kinase activities. In cancer, unregulated tyrosine kinase activity can drive tumor malignancy and formation by generating inappropriate proliferation and survival signals. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to these studies. The anti-phosphotyrosine rabbit polyclonal antibody developed by PTM Biolabs, Inc. provides an exceptionally sensitive tool for studying tyrosine phosphorylation and its kinase activities in biomedical and preclinic studies as well as high throughput drug discovery.


    Storage & Stability

    Store product at -20oC. Avoid repeated freeze/thaw. Antibody is supplied in PBS with 50% glycerol and 0.01% sodium azide.Stable for 12 months from date of receipt.


    **Research purposes only. Not intended to be used for therapeutic or diagnostic purposes in humans or animals.



  • 技术原理

    Cat#:

    PTM-702

    Ab Type:

    P

    Applications:

    WB

    Species:

    All

    Price: (100 μl)

    2880.00

    Price: (20 μl)

    864.00


  • 仪器设备

  • 技术优势

  • 应用领域

    ELISA, WB, IAP. Not tested in other applications

    *WB=Western blotting; IAP: Immuno affinity precipitation


  • 参考文献

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