修饰抗体 Products
赖氨酸巴豆酰化 (crotonylation)抗体
杭州景杰生物科技有限公司
Anti-lactyllysine rabbit pAb

Cat#:

PTM-1401

Ab Type:

P

Applications:

WB

Species:

All


Product Description

Anti-lactyllysine rabbit pAb

Anti-lactyllysine rabbit pAb

Figure A: Dot blotting analysis on indicated amount of peptides using anti-Kla rabbit pAb. The list of peptides is included in the table on the right

Figure B:  Western blotting analysis on 30 μg of crude proteins from  HeLa whole cell with (+) or without (-) treatment of sodium lactate (100 mM, 24hr), 30μg whole  tissue lysates of mouse kidney and mouse spleen using anti-Kla rabbit pAb (1: 1000).

Figure C: Western blotting analysis on 30 μg of crude proteins from human HeLa, mouse N2a and Rat BRL whole cell lysates using anti-Kla rabbit pAb (1: 1000)


Source/Purification

This product is produced by immunizing rabbits with lactylated BSA.

Antibodies are purified by lactylated-lysine agarose affinity chromatography.



Specificity

All crotonylated proteins expected

Recommended Applications

ELISA, Dot blot, Western blot.  Recommended antibody dilution: WB: 1:1000


NOTE: For WB, incubate membrane with diluted antibody in 5% nonfat milk, 1 x TBS, 0.1% Tween-20 for two hours at room temperature with gentle shaking. Prepare working dilution immediately before use. Use at an assay dependent concentration. Optimal dilutions/concentrations should be determined by the end user. Not yet tested in other applications


 




Scientific Description

Histones are subjected to a variety of enzyme catalyzed modifications, including acetylation, methylation, phosphorylation, ubiquitylation, etc. The Warburg effect, which originally described increased production of lactate in cancer, is associated with diverse cellular processes such as angiogenesis, hypoxia, polarization of macrophages and activation of T cells. This phenomenon is intimately linked to several diseases including neoplasia, sepsis and autoimmune diseases. Lactate, which is converted from pyruvate in tumour cells, is widely known as an energy source and metabolic by-product. Zhang et al. (Nature, 2019) reveal that histone lactylation is a previously unknown histone modification, derived from the cellular metabolite lactate.




Storage & Stability

The antibody is kept in PBS with 50% glycerol and 0.01% sodium azide. Upon receipt, please centrifuge the antibody at 12,000 x g for 20 seconds and store the antibody at -20°C. Avoid repeated freeze/thaw cycles. Stable for 12 months from date of receipt. Leave the antibody at room temperature for 2 minutes and gently mixed using pipette before usage.




Zhang D, Tang ZY. et al (2019). Metabolic regulation of gene expression by histone lactylation. Nature, vol. 574, pages 575–580 .

ELISA, WB, IAP. Not tested in other applications

*WB=Western blotting; IAP: Immuno affinity precipitation


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